We have developed a molecular PA imaging system capable of visualizing PSMA molecular contrast in vivo. We have designed a series of PA imaging contrast agents composed of three functional parts (Figure 1). The middle component is a linker that tethers the PSMA ligand to bulky optical dyes such as IRDye800CW, ICG, and Alexafluor750, which…
Tag: advanced ultrasound imaging
The feasibility of transcranial functional PA imaging of cerebral oxygenation in the piglet brain (5-day old, 2kg) was demonstrated (Figure 1a). Multi-spectral PA data were collected (700-900 nm in 10-nm intervals) while applying various fractional inspired oxygen (FiO2) levels to induce graded hypoxia. The ground-truth sO2 was also measured by direct sampling of venous blood…
PA imaging has a potential to sense the electrophysiological state of biological tissue. Voltage sensitive dyes are designed to monitor membrane potential by detecting fluorescence changes in response to neuronal or muscle electrical activity. Through our collaboration with Prof. Les Loew (University of Connecticut) who pioneered VSD synthesis, we have reported the first PA VSD…
For clinical diagnosis and surgical monitoring and guidance, many imaging modalities are able to non-invasively show regional anatomy and position details, but few of them are able to provide functional information. Monitoring the concentration of metabolism-related species or bio-markers could also be helpful during surgery and in interventional imaging. For example, to determine whether the…
H. K. Zhang, Y. Chen, J. Kang, A. Lisok, M. G. Pomper, E. M. Boctor, “Prostate specific membrane antigen-targeted photoacoustic imaging of prostate cancer in vivo,” Journal of Biophotonics 11(9), e201800021, 1 Sep. 2018 Y. Wu, W. Lesniak, J. Kang, S. Ray, M. G. Pomper, E. M. Boctor, “Multispectral photoacoustic imaging of aggressive prostate cancer in vivo using PAMAM dendrimer conjugated agent,” IEEE International…
J. Kang, R. C. Kohler, S. Adams, E. M. Graham, and E. M. Boctor, “Light-emitting diode-based transcranial photoacoustic measurement of sagittal sinus oxyhemoglobin saturation in hypoxic neonatal piglets,” bioRxiv 262451, 24 Aug. 2020 J. Kang, E. M. Boctor, S. Adams, E. Kulikowicz, H. K. Zhang, R. C Koehler, E. M Graham “Validation of noninvasive photoacoustic measurements of…
J. Kang, S. D. Kadam, J. Elmore, B. J. Sullivan, H. Valentine, A. P. Malla, M. M. Harraz, A. Rahmim, J. U. Kang, L. M. Loew, M. Baumann, A. A. Grace, A. Gjedde, E. M. Boctor, and D. F. Wong, “Transcranial photoacoustic imaging of NMDA-evoked focal circuit dynamics in rat hippocampus,” Journal of Neural Engineering 17(2), 025001, 8…
The current technique for prostate cancer detection is B-mode transrectal ultrasound (TRUS) guided systematic core needle biopsy. However, due to the limited sensitivity and capability of categorizing aggressiveness of prostate cancer in B-mode images, additional imaging modality such as magnetic resonance imaging (MRI) is necessary to support targeted biopsies. To reduce the imaging cost, we…